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Spin column type of product for separation/purification up to 20ug of high purity Plasmid DNA
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Up to 20ug high yield and high purity of plasmid DNA can be obtained by separation and purification with high reproducibility.
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Separation and purification for up to 30 kb of plasmid DNA
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Containing Lysis & Neutralization indicator (Lysis Viewer)
DNA-Spin™ Plasmid DNA Purification Kit is able to perform separation and purification for high concentrated, purity plasmid DNA from bacteria using silica membrane and optimized alkali lysis buffering system. Neutralization buffering system used for pH neutralization and binding perform not only separation/purification for plasmid DNA over 30Kb, but also remove chromosomal DNA, cell debris effectively after lysis. Also, pH change through alkaline lysis method can be observed visually in real time because the product contains lysis & neutralization indicator such as Lysis Viewer. Product is used for either High-copy plasmid DNA or low-copy plasmid DNA and in order to perform separation and purification effectively from endA+ strain(HB101, the JM strains, NM series strains, PR series strains, etc.), Washing Buffer A is provided separately.
Ordering Information:
INTRON#17098 DNA-spin™ Plasmid DNA Purification Kit, 200 Col.
Product available for performing 65 bp ~ 10 Kb size of DNA extraction and clean up from PCR product and agarose gel
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Recovery of DNA is very high
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PCR clean up : 90 ~ 95%
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Gel extraction : 70 ~ 85%
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Experiment is very simple and quick
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Available for downstream applications such as sequencing and restriction enzyme digestion
MEGAquick-spinTM Plus Total Fragment DNA Purification Kit is used for performing separation/purification of Fragment DNA from PCR product, agarose gel DNA and DNA Clean-up. 65 bp to 100Kb size of DNA can be separated/purified. Furthermore, DNA is purified effectively while removing almost all primer dimer in PCR product. Also, high yield of DNA can be obtained by performing lysis for agarose gel with various concentration. Downstream applications such as DNA sequencing, cloning, labeling, restriction enzyme digestion can be conducted by using high yield of purified DNA.
Ordering Information:
INTRON#17290 MEGAquick-spin™ Plus Total Fragment DNA Purification Kit, 200 Col.
RedSafe Nucleic Acid Staining Solution
RedSafe Nucleic Acid Staining Solution is a new and safe nucleic acid stain, an alternative to the traditional ethidium bromide(EtBr) stain for detecting nucleic acid in agarose gels. It emits green fluorescence when bound to DNA or RNA. This new stain has two fluorescence excitation maxima when bound to nucleic acid, one centered at 309 nm and another at 419 nm. In addition, it has one visible excitation at 514 nm. The fluorescence emission of RedSafe bound to DNA is centered at 537 nm.
RedSafe Nucleic Acid Staining Solution is as sensitive as EtBr. The staining protocol for RedSafe Nucleic Acid Staining Solution is similar to that for EtBr. Compared to EtBr, known as a strong mutagen, RedSafe Nucleic Acid Staining Solution causes much fewer mutations in the Ames test. In addition, RedSafe Nucleic Acid Staining Solution has a negative result in mouse marrow chromophilous erythrocyte micronucleus test and mouse spermary spermatocyte chromosomal aberration test. So it is wise to choose RedSafe Nucleic acid Stainig Solution instead of EtBr for detecting nucleic acid in agarose gels.
Characteristics
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Used for detecting DNA and RNA.
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Alternative to the ethidium bromide staining.
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As sensitive as EtBr.
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Non-toxic, non-mutagenic and non-carcinogenic.
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No hazard waste.
Gel analysis of different size of PCR products using RedSafe TM Nucleic Acid Staining Solutin (20,000x)
Ordering Information:
INTRON#21141 RedSafeTM Nucleic Acid Staining Solution(20,000X), 1mL