Service includes:
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Design Materials and Methods
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Data Generation and Analysis
e.g. Gene Expression, Gene Synthesis, Mutation Analysis, Bacterial / Fungal / Viral Identification
Low price, double-strand DNA fragments deliver in few working days!
DNA Fragment Size
Delivery Time
Less than 500 bp
5 - 7 working days
501 - 600 bp
6 - 8 working days
601 - 700 bp
701 - 800 bp
801 - 900 bp
901 - 1,000 bp
More than 1,000 bp
On Request
Product provided
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0.25 –1ug of lyophilized PCR product.
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Blunt-ended double stranded DNA.
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Protocol for adding the adenosine (A) overhangs to blunt-ended gene fragments on request.
Applications
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Suitable for gene construction or modification, antibody research, standard in qPCR assay, control for PCR assay and cloning.
Ordering:
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Please send your gene sequence to info@techdragon.com.hk
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Gene sequence contains of difficult synthesis regions (e.g. high or low GC content, repetitive sequence, homo- polymeric tracts) may be rejected.
Biosafety and regulatory check
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A biohazard disclosure statement is required.
Service Includes
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Double strand oligo synthesis
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Cloning of double strand oligo into vector
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Oligo sequence is confirmed by Sanger Sequencing or NGS
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Delivery time: 10 - 30 working days, depending on gene length and complexity
Package Includes
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2 - 5 ug purified plasmid
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Sanger Sequencing or NGS result
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High throughput DNA/RNA extraction using chemagic Magnetic Separation Module I (chemagic MSMI) automated nucleic acid isolation system.
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A Complete solution for preparation of nucleic acids from various sample materials, such as blood, tissue, bacteria, food, plants, etc.
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The DNA/RNA obtained can be utilized in comprehensive molecular and genomics applications, including PCR, Real-time PCR, Sequencing, Next Generation Sequencing, Microarray, SNP genotyping and Pathogen Detection.
Service
Sample Volume
Throughput
Genomic DNA from whole blood
200 ul
400 samples/day
Viral DNA/RNA from plasma
200 ul
400 samples/day
Genomic DNA from whole blood
4 ml
100 samples/day
Viral DNA/RNA from plasma
2 ml
100 samples/day
Service includes
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Conversion of Conventional PCR to Real-Time PCR
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Copy Number Determination / Gene Expression Profiling
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Genotyping / Allelic Discrimination
Sizing PCR fragments for...
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Linkage Mapping
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Microsatellite analysis
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Short Tandem Repeat (STR)
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16S Microbial Identification Scans (tRFLP)
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Amplified Fragment Length Polymorphism (AFLP)
Details
Size Standard Provided
GeneScan 400HD - ROX
GeneScan 500 - ROX
GeneScan 500 - LIZ
Fragment size
50 - 400 bp
35 - 500 bp
35 - 500 bp
Sample dye
FAM, VIC & NED
FAM, VIC & NED
FAM, VIC, NED & PET
Size standard dye
ROX
ROX
LIZ
Sample Preparation
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5 ul of fluorescent labeled PCR product in 8-well strip PCR tube or 96-well plate sealing with 8-well strip cap. Please don't use PCR sealing film or foil to seal the plate. Concentration of products needs to be optimized by the client.
Data Delivery
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.fsa file will be delivered by e-mail.
Turnaround Time
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2 - 4 working days
Custom Service
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Other fragment sizes and tRFLP products purification are on request.
Confidentiality
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We guarantee to maintain the confidentiality of your data at any time. Your complete data will be deleted after 30 days and residual samples will be dismissed after 7 days.
Remarks
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Minimum order of 20 reactions per batch
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Size standard will be provided for free
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Delivery of data is dependent on sample quality provided
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Full price will be charged for failed samples