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Address:

Room 808, Topsail Plaza, 11 On Sum Street, Shatin, N.T., Hong Kong

Tel : (852) 2646 5368      Fax : (852) 2646 5037

Email : info@techdragon.com.hk

 

地址:

香港 沙田 安心街11號 華順廣場 8 樓 808 室

電話: (852) 2646 5368     傳真: (852) 2646 5037

電郵: info@techdragon.com.hk

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DNA SEQUENCING

 

Sample Preparation Guideline

 Tube Format

  • Use 1.5ml micro-centrifuge tube or 8-well strip tube (sealed with 8-well strip cap) for DNA template and primer.   

  • DNA template for more than 1 read, please provide individual tube for each read.  

 

Plate Format 

  • Use 8-well strip tube or 96-well plate. To avoid evaporation or leaking, please use the 8-well strip cap to seal plate.

 

Standard Sequencing Service (SS)         
   DNA Template and primer in individual tube    

   DNA Template - Primer Pre-mix

         

 

 

 

PCR Product Sequencing Service (PS)    

  • Unpurified PCR product:  20 ul

  • Send your specific primer: 10 ul in 10 pmol/ul (10uM) per 10 reads.

  • Please provide gel photo.

  • Template-Primer Premix is not accepted.

PCR Product Extraction from Agarose Gel    

  • Use 1.5ml micro-centrifuge tube.

  • Maximum 300 mg of 1% agarose gel or 150 mg of 2 % agarose gel.

  • Send your specific primer: 10 ul in 10 pmol/ul (10uM) per 10 reads.

  • Please provide gel photo.

Difficult Template Sequencing Service (DS)

  • Unpurified PCR product:  20 ul PCR reaction

  • Gel extraction sample:  Maximum 300 mg of 1% gel or 150 gm of 2 % gel.

  • Send your specific primer: 10 ul in 10 pmol/ul (10uM) per 10 reads.

  • Template-Primer Premix is not accepted.