DNA SEQUENCING
Sample Preparation Guideline
Premixed Service (PM)
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DNA Template and primer are premixed according to Table 1.
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Please use 1.5ml microcentrifuge tube (snap cap) or 8-tube strip (with 8-cap strip).
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Do not accept: 2 ml, 0.5 ml and 0.2 ml single tube and screw cap tube.
Table 1 DNA template and Primer concentration for Premixed Service
Premixed - Plate Format
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Use 8-well strip tube or 96-well plate. To avoid evaporation or leaking, please use the 8-well strip cap to seal plate.
Standard Service (SS)
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DNA Template and primer in separated tubes according to Table 2.
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Please use 1.5 ml microcentrifuge tube (snap cap) for template and primer. Other tubes are not accepted.
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One tube of template for each reaction. Additional charge for aliquot of template.
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Send your specific primer: 10 ul in 10 pmol/ul (10uM) per 10 reactions.
Table 2 DNA template and Primer concentration for Standard Service
Tube Labelling
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·Label sample tubes with number correspond to the order form. Do not to put the template name on the tube.
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·Fill one template and one primer in each line. If the template need to use more than one primer, please fill in separate lines.
Label tube as "1", "2", "3".
Label tube as "1", "2", "3", "4".
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·Do not fill in more than one primer in one line.
Difficult Template Sequencing Service (DS)
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NA Template and primer in separated tubes according to Table 3.D
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Use 1.5ml tube for template and primer.
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Template - Primer Premixed format is not accepted.
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Please provide gel photo.
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Send your specific primer: 10 ul in 10 pmol/ul (10uM) per 10 reads.