Next Generation Sequencing Services
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Sample Preparation Guidelines for Applied Biosystems' SOLiD |
The following guidelines are designed to give you a basic idea of how to prepare your samples. However, not every application can be covered here and each project is different. So, please contact us for your project specific criteria.
Fragment Library-based Projects:
¡P DNA must be double-stranded
¡P Fragments must be > 500bp
¡P Avoid whole genome amplification methods whenever possible
¡P For fragment libraries of low complexity organisms, we recommend submitting 1-4£gg of DNA at concentration of
100ng/£gl
¡P For fragment libraries of high complexity organisms, we recommend submitting 4-20£gg of DNA or more, at a
concentration of 500ng/£gl
¡P DNA should have an OD260/280 ratio of 1.8 or more
¡P Please provide a 1-2% agarose gel image of your sample with a 1kb ladder
¡P DNA should not be degraded, nor should it contain any particulate matter
Mate-paired Library-based Projects:
¡P DNA must be double-stranded
¡P Avoid whole genome amplification methods whenever possible
¡P For mate-paired libraries of low complexity organisms, we recommend submitting 10-20£gg of DNA at concentration
of 1£gg/£gl
¡P For mate-paired libraries of high complexity organisms, we recommend submitting 50-100£gg of DNA or more, at a
concentration of 1£gg/£gl
¡P DNA should have an OD260/280 ratio of 1.8 or more
¡P Please provide a 1-2% agarose gel image of your sample with a 1kb ladder
¡P DNA should not be degraded, nor should it contain any particulate matter
Transcriptome or small RNA-based Projects:
¡P Please contact us for guidelines.