Methylation Analysis

Using the DNA sequencing method to detect methylated Cytosine is the gold standard for bisulfite techniques.  Tech Dragon offers the comprehensive service from DNA extraction to data analysis for detecting the presence of Cytosine methylation.

Methylation Bisulfite Service includes:

·      Genomic DNA extraction

·      Preliminary PCR and sequencing of genomic DNA

·      Bisulfite conversion of unmethylated cytosines

·      Methylation Specific Primer design and validation

·      Optimized PCR amplification of bisulfite treated target DNA

·      Direct sequencing of PCR products

·      Cloning of PCR products for sequencing

·      Data Analysis