Difficult Template Sequencing Service                   Order Form

 

 

In DNA sequencing, there are some templates/regions that are difficult to sequence when using the standard DNA sequencing protocol.  These difficult templates form secondary structures that cause unsatisfactory sequencing results.  The problem cannot be solved even when DMSO is added to the sequencing reaction mix.  Difficult templates include:

 

 

Tech Dragon employs different protocols with field proven sequencing enhancers to sequence these difficult templates. With a clone or unpurified PCR product, we can purify the template and perform sequencing using these proprietary protocols.

 

 

Bacterial Clone or Plasmid DNA

 

Sample Required:	1 mL bacterial culture or 10 ug plasmid DNA in 1.5 mL tube Specific primer: 10 ul in 10 pmole/ul in 1.5 mL tube
Service:	· Plasmid preparation · Standard vector primers are provided for free · Using 3 different optimized protocols (for one primer) · The best sequencing result will be delivered by e-mail · Turnaround time: 1 - 2 weeks.

 

 

PCR Product

 

Sample Required:	25 - 50ul of unpurified PCR product or 200 - 400 mg gel slice Specific primer: 10 ul in 10 pmole/ul in 1.5 mL tube
Service:	· PCR product purification · Using 3 different optimized protocols (for one primer) · The best sequencing result will be delivered by e-mail · Turnaround time: 1 - 2 weeks