Next Generation Sequencing Services

Whole Genome Resequencing

 

The SOLiD System enables a new level of whole genome sequencing with massive throughput and mate-paired technology.  The system's ultra high throughput results in high coverage levels and the ability to sequence multiple genomes in a single run.  The combination of unmatched throughput and superior accuracy dramatically reduces the time and cost to lower than that of other technologies.

Targeted Resequencing

 

The superior accuracy of the SOLiD System provides a very low false positive rate that enables variation detection with less coverage than alternative platforms.  The inclusion of barcodes for sample multiplexing dramatically lowers the cost, time and labour involved in processing multiple samples.

Small RNA Analysis

 

The SOLiD Small RNA Solution is a robust method for hypothesis-generating, whole genome analysis of expression patterns that enable discovery of novel RNA without the probe bias of microarrays.  Using the SOLiD Small RNA Expression Kit, you can convert total RNA into a library suitable for emulsion PCR using five easy steps with a single purification. This simplified protocol conserves the strand information of the original molecule and enables one to decipher strand-specific expression patterns.

Whole Transcriptome Analysis

 

The SOLiD System provides researchers with access to the entire transcriptome with a hypothesis generating, highly sensitive assay that preserves the strand information of the original RNA molecule. The combination of 400 M sequence tags and sample multiplexing capabilities allow cost effective analysis of multiple samples in a single run while maintaining the sensitivity to discover molecules present at low levels.

De Novo Sequencing

The longer read lengths and new data analysis software of the SOLiD System enable the de novo assembly of small organisms. Researchers have demonstrated the assembly of 50 bp reads into a scaffold that can then be filled in with capillary electrophoresis-based sequencing.

 

Gene Expression Profiling

 

The massive amount of sequence tags generated in a single run can be used to identify and quantify exon sequences associated with alternative splicing and promoter usage.  Simple tag counting allows for digital readout of expression levels and the ability to detect lowly expressed genes with greater sensitivity.

Chromatin Immunoprecipitation (ChIP)

 

ChIP-Sequencing (ChIP-Seq) with the SOLiD System combines a chromatin immunoprecipitation (ChIP) assay with massively parallel DNA sequencing, offering a highly sensitive method to accurately characterize the protein-DNA interactions of an entire genome.  Additionally, the SOLiD System's flexible slide format and multiplexing capability allows researchers to analyze multiple samples in a single run.

Methylation Analysis

 

The SOLiD System provides a high throughput method for analyzing genome-wide DNA methylation with a much higher level of resolution than traditional methods.